Recommended Name ?endolysin
Systematic Name ?peptidoglycan amidohydrolase
Alternative Name ?endolysin Ply3626
acetylmuramoyl-alanine amidase
acetylmuramyl-alanine amidase
acetylmuramyl-L-alanine amidase
N-acetylmuramic acid L-alanine amidase
N-acetylmuramyl-L-alanine amidase
N-acetylmuramylalanine amidase
N-acylmuramyl-L-alanine amidase
peptidoglycan amidohydrolase
Uniprot IDQ8SBN4
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?NAM amidase
Reaction ?1. Catalysis of the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides (R04112)
    Corresponding Pfam domain: Amidase_3
    Evidence: predicted (PubMed: 12406719)
Source Organism ?Clostridium phage phi3626
Target Organism ?Clostridium perfringens
Clostridium fallax
Disease ?Food poisoning (Clostridium perfringens)
Gas gangrene (Clostridium perfringens)
Gas oedema in animals (Clostridium fallax)
Necrotizing fasciitis (Clostridium perfringens)
State ?Tested
Reference ?12406719

Studies found: 1

Antimicrobial Agent
Study Type
AdministrationRelevant ResultsAdverse Effects and Other IssuesReference ?
Ply3626 in vitro
  • set of 48 Clostridium perfringens strains
  • set of 30 strains of various other Clostridium species
  • set of 34 bacterial strains belonging to other genera
Efficient lytic activity against all 48 strains tested; lysin exhibited stringent specificity for C. perfigens, besides only C. fallax was found to be lysis sensitive (probably due to the presence of same type of peptidoglycan). Other Issues
For efficient production of endolysin, unusual codone composition due to low GC content of ply3626 must be compensated.