Recommended Name ?N-acetylmuramoyl-L-alanine amidase
Systematic Name ?peptidoglycan amidohydrolase
Alternative Name ?endolysin PlyV12
acetylmuramoyl-alanine amidase
acetylmuramyl-alanine amidase
acetylmuramyl-L-alanine amidase
N-acetylmuramic acid L-alanine amidase
N-acetylmuramyl-L-alanine amidase
N-acetylmuramylalanine amidase
N-acylmuramyl-L-alanine amidase
peptidoglycan amidohydrolase
Uniprot IDQ6E4B1
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?NAM amidase
Reaction ?1. Catalysis of the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides (R04112)
    Corresponding Pfam domain: Amidase_5
    Evidence: predicted (PubMed: 15231813)
Source Organism ?Enterococcus sp. phage 1
Target Organism ?Enterococcus faecalis
Enterococcus faecium
Streptococcus pyogenes
Streptococcus agalactiae
Note on TargetS. pyogenes is also known as Group A Streptococcus.S. agalactiae is also known as Group B Streptococcus.PlyV12 also target Group C, E and G streptococci.
Disease ?Bacteremia (Enterococcus faecium; faecalis)
Infective endocarditis (Enterococcus faecium; faecalis)
Neonatal sepsis (Group B Streptococcus)
Nosocomial infections (Enterococcus faecium; faecalis)
Postpartum infections (Group B Streptococcus)
Urinary track infections, Females (Enterococcus faecium; faecalis)
Urinary track infections, Males (Enterococcus faecium; faecalis)
Wound infections (Enterococcus faecium; faecalis)
State ?Tested
Reference ?15231813

Studies found: 1

Antimicrobial Agent
Study Type
AdministrationRelevant ResultsAdverse Effects and Other IssuesReference ?
PlyV12 in vitro
  • set of 15 clinical and laboratory strains of Enterococcus faecalis, Enterococcus faecium including 5 VRE strains
  • set of Lancefield group streptococci
  • set of other streptococci
  • set of staphylococci
Efficient lytic activity against all 15 E. faecium and E. faecalis strains tested (including VR strains).

Significant lytic activity against pathogenic streptococci including Streptococcus pyogens and Lancefield groups B, C, E and G streptococci.

Low lytic activity against Staphylococcus aureus and some commensal bacteria.
Other Issues
Despite the fact that lysin was found to have maximal activity at about pH 6.0, under physiological conditions (pH 7.4) no deterioration of lysin activity was observed.