Protein 17

Recommended Name ?Amidase
Alternative Name ?S. aureus phage P68 virion muramidase
Uniprot IDQ859I7
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?Other amidase/peptidase
Reaction ?1. Catalysis of the hydrolysis of the bonds in cell wall peptidoglycans (amidohydrolase/peptidase activity)
    EC: n/a
    Corresponding Pfam domain: CHAP
    Evidence: predicted
Source Organism ?Staphylococcus phage P68
Target Organism ?Staphylococcus aureus
Disease ?Infective endocarditis
Nosocomial infections
State ?Tested
Reference ?15980371

Studies found: 1

Antimicrobial Agent
Study Type
AdministrationRelevant ResultsAdverse Effects and Other IssuesReference ?
Protein 17 in vitro
  • Staphylococcus aureus 68 and 35 clicnical isolates of S. aureus, 16 of them methicillin resistant.
Antimicrobial activity of protein 17 was tested on various S. aureus 68. 1x107 CFU of exponentially growing S. aureus was exposed to to different concentrations (5pg – 5µg/ml) of purified protein 17 for 20 min. From these results, the 50% inhibitory concentration for protein 17 was calculated to be 500 pg/ml.

Protein 17 was further tested on 35 clicnical isolates of S. aureus. Purified protein 17 (50ng/ml) was added to exponentially growing cells (1x107 CFU). After 20 min exposure, 99% of the majority of the S. aureus isolates was reduced.
Other Issues
Protein 17 displayed activity also against S. aureus strains resistant to phage P68 infection. This suggests that the bacterial receptor required for phage attachment differs from the ligand required for protein 17 binding.