phi11 endolysin

Recommended Name ?amidase
Systematic Name ?peptidoglycan amidohydrolase
Alternative Name ?acetylmuramoyl-alanine amidase
acetylmuramyl-alanine amidase
acetylmuramyl-L-alanine amidase
N-acetylmuramic acid L-alanine amidase
N-acetylmuramyl-L-alanine amidase
N-acetylmuramylalanine amidase
N-acylmuramyl-L-alanine amidase
peptidoglycan amidohydrolase
Uniprot IDQ8SDS7
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?NAM amidase
Reaction ?1. Catalysis of the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides (R04112)
    Corresponding Pfam domain: Amidase_2
    Evidence: experimental (PubMed: 10336488)
2. Catalysis of the hydrolysis of the peptide bond between D-alanine and glycine in cell wall peptidoglycans
    EC: n/a
    Corresponding Pfam domain: CHAP
    Evidence: experimental (PubMed: 10336488)
Source Organism ?Staphylococcus phage 11
Target Organism ?Staphylococcus aureus
Note on TargetThe phi11 endolysin also target coagulase-negative staphylococci.
Disease ?Infective endocarditis
Nosocomial infections
State ?Tested
Reference ?10336488

Studies found: 2

Antimicrobial Agent
Study Type
AdministrationRelevant ResultsAdverse Effects and Other IssuesReference ?
phi11 endolysin in vitro
  • set of staphylococcal mastitis pathogens including Staphylococcus aureus (ATCC 29740) and coagulase-negative staphylocooci:
    Staphylococcus agalactiae
    Staphylococcus chromogenes
    Staphylococcus epidermidis
    Staphylococcus hyicus
    Staphylococcus simulans
    Staphylococcus warneri
    Staphylococcus xylosus
Efficient lytic activity against all major staphylococcal mastitis pathogens with exception for S. agalactiae.

Other Issues
Any removal of either of the two enzymatic domains decreased the efficiency of bacterial lysis. Lysin retained its activity at the physiological pH calcium concentration found in milk.
phi11 endolysin in vitro
  • set of staphylococci including:
    Staphylococcus aureus NCTC8325
    Staphylococcus aureus Wood 46
    Staphylococcus aureus Cowan 1
    Staphylococcus aureus Newman
    Staphylococcus epidermidis O-47
    Staphylococcus simulans 22
Efficient lytic activity against the staphylococcal cells of the all strains tested with lytic activity essentially comparable to that of lysostaphin.
Lysin was also capable of elimination the biofilm of S. aureus, but not that of S. epidermidis.
Other Issues
Any removal of either enzymatic domains decresed the efficiency of bacterial lysis, whereas deletion of cell wall binding domain resulted in complete loss of the lytic activity.