phage B30 lysin

Recommended Name ?peptidoglycan endolysin
Systematic Name ?peptidoglycan N-acetylmuramoylhydrolase
Alternative Name ?GBS bacteriophage B30 lysin
mucopeptide glucohydrolase
mucopeptide N-acetylmuramoylhydrolase
peptidoglycan N-acetylmuramoylhydrolase
Uniprot IDQ8HA43
General Mode of ActionEnzymatic cleavage of peptidoglycan which results in a rapid lysis of the bacterial cell.
phiBIOTICS Family ?Lysozyme
Reaction ?1. Catalysis of the hydrolysis of the beta-(1->4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
    Corresponding Pfam domain: Glyco_hydro_25
    Evidence: experimental (PubMed: 15256551)
2. Catalysis of the hydrolysis of the peptide bond between D-alanine and L-alanine in cell wall peptidoglycans
    EC: n/a
    Corresponding Pfam domain: CHAP
    Evidence: experimental (PubMed: 15256551)
Source Organism ?Streptococcus phage B30
Target Organism ?Streptococcus agalactiae
Streptococcus pyogenes
Note on TargetS. pyogenes is also known as Group A Streptococcus.S. agalactiae is also known as Group B Streptococcus.Phage B30 lysin also target Group C, E and G streptococci.
Disease ?Acute glomerulonephritis (Group A Streptococcus)
Acute rheumatic fever (Group A Streptococcus)
Impetigo (Group A Streptococcus)
Infective endocarditis (Group A Streptococcus)
Neonatal sepsis (Group B Streptococcus)
Pharyngitis (Group A Streptococcus)
Postpartum infections (Group B Streptococcus)
Rheumatic heart disease (Group A Streptococcus)
Scarlet fever (Group A Streptococcus)
State ?Tested
Reference ?15256551

Studies found: 2

Antimicrobial Agent
Study Type
AdministrationRelevant ResultsAdverse Effects and Other IssuesReference ?
phage B30 lysin in vitro
  • set of Lancefield group streptococci
  • set of other streptococci
  • set of lactobacilli
  • Staphylococcus aureus
Efficient lytic activity against Streptococcus agalactiae (group B); groups A, C, E, and G streptococci.

Enterococcus faecalis and autolysis-negative mutant of Streptococcus pneumonie (LytA-) were lysed very slowly.

No lytic activity against lactobacilli or S. aureus were observed.
Other Issues
The pH optimum for lysin is between 5.5 and 6.0, somewhat higher then cited normal vaginal pH (4.5). In this case lysin may be most effective in clearing heavy GBS vaginal colonization (due to considerably higher vaginal pH in women who are heavily colonized with GBS).
phage B30 lysin in vitro
  • set of streptococci including:
    Streptococcus agalactiae
    Streptococcus uberis
    Streptococcus salivarius
  • set of other Gram-positive bacteria including:
    Staphylococcus aureus
Efficient lytic activity against S. agalactiae, S. uberis and S. aureus. 16598006